Table of Contents
Title Page
About this document
Introduction
Optical Tweezers
Timeline: Optical Tweezers and their
application to Molecular Motors
Types of optical trap
Building an optical trap
Apparatus schematic
Apparatus components
Acousto Optic Deflectors
Position Sensors
Trapping theory
Thermal Motion of Trapped Objects
Restoring Force
Thermal Energy
Damping
Power Spectrum
Trap Stiffness
Calibration
TIRF
TIRF Theory
Microscope configurations for TIRF
TIRF in practice
TIRF Application Examples
Actomyosin
Background
Motility Assays
Trapping
Optical Tweezers Simulation
TIRF
Conclusion
Glossary
Bibliography
